Monodisperse Polystyrene (PS) Microspheres – Size Standard for SEM, Fl

Monodisperse Polystyrene (PS) Microspheres – Size Standard for SEM, Flow Cytometry & DLS Calibration | PolyRef™

100 nm

$79.00

Monodisperse Polystyrene (PS) Microspheres – Size Standard for SEM, Flow Cytometry & DLS Calibration | PolyRef™

$79.00

Particle Size 100 nm 200 nm 500 nm 1 μm 2 μm 3 μm 5 μm 10 μm

Product Overview

PolyRef™ Monodisperse Polystyrene Microspheres are the workhorse particle size standard for scientific research. Each batch is manufactured under strict process control to achieve narrow size distribution (CV ≤ 6% for sizes ≥ 100 nm) and verified by SEM, TEM, optical microscopy, and laser diffraction. Available in selected sizes from 100 nm to 10 μm; full range from 20 nm to 1000 μm available on request.

Particles are dispersed in ultrapure water at 2.5 wt% (25 mg/mL) solids content. Sub-5 μm particles are non-crosslinked monodisperse PS; particles ≥ 5 μm are lightly crosslinked for enhanced mechanical and chemical stability.

Why PolyRef™ PS Microspheres?

Tight monodispersity. CV ≤ 6% across 100 nm – 10 μm range; CV ≤ 10–20% for sub-100 nm sizes.
Batch-traceable QC. Every batch includes SEM imaging and size distribution data; COA shipped with each order.
Full size coverage on request. 20 nm to 1000 μm — over 50 size grades available for custom orders.
Cited in peer-reviewed journals. RIGOR Science microspheres have been cited in Nature Water, Nature Sustainability, Advanced Science, and others.
Direct from the manufacturer. No distributor markup. Custom sizes, surface modifications, and concentrations available.

Specifications

Material	Polystyrene (PS) — non-crosslinked (≤ 5 μm) or lightly crosslinked (≥ 5 μm)
CAS No.	9003-53-6
Chemical Formula	(C₈H₈)ₙ
Density	1.05–1.07 g/cm³
Refractive Index	1.59 (at 589 nm)
Surface	Plain (hydrophobic); amino / carboxyl / fluorescent versions available separately
Size Uniformity (CV)	≤ 6% (100 nm – 1000 μm); ≤ 10–20% (20–80 nm)
Available Sizes (Stock)	100 nm, 200 nm, 500 nm, 1 μm, 2 μm, 3 μm, 5 μm, 10 μm
Custom Sizes	20 nm – 1000 μm available on request
Concentration	2.5 wt% (25 mg/mL); 5 wt% / 10 wt% / dry powder available on request
Dispersion	Ultrapure water
Package Size	10 mL (5 mL, 50 mL, 200 mL on request)
Storage	2–8 °C for suspensions; room temperature (dry, dark) for powders
Shelf Life	60 months
Characterization	SEM, TEM, optical microscopy, laser diffraction; batch-specific COA included

Applications

Instrument calibration — SEM, flow cytometry, DLS, NTA, laser diffraction, optical microscopy
Particle size reference standards and QC
Photonic crystal self-assembly and templating
LCD spacer applications
Light-scattering and diffusing agents
Porous material templating (sacrificial templating)
Hydrodynamic and microfluidic flow tracing
Cell-size mimics for membrane and uptake studies (5–10 μm)
Drug delivery carrier model systems
Microplastic spectral reference (when used alongside PVC / LDPE / PET / PMMA standards)

Custom Specifications

Need a size, concentration, or surface chemistry not listed above? RIGOR Science manufactures the full 20 nm – 1000 μm range and offers custom concentrations (5%, 10%, dry powder), packaging sizes (5 mL to 200 mL), and surface functionalization (amino, carboxyl, sulfate, fluorescent). Contact us with your specifications.

Sub-micron grades (100–200 nm) are characterized by DLS; micron grades (≥1 μm) are characterized by laser diffraction / optical microscopy. Representative data shown; see COA for lot-specific values.