{"product_id":"monodisperse-polystyrene-ps-microspheres-size-standard-for-sem-flow-cytometry-dls-calibration-polyref™","title":"Monodisperse Polystyrene (PS) Microspheres – Size Standard for SEM, Flow Cytometry \u0026 DLS Calibration | PolyRef™","description":"\u003ch3\u003eProduct Overview\u003c\/h3\u003e\n\u003cp\u003e\u003cstrong\u003ePolyRef™ Monodisperse Polystyrene Microspheres\u003c\/strong\u003e are the workhorse particle size standard for scientific research. Each batch is manufactured under strict process control to achieve narrow size distribution (CV ≤ 6% for sizes ≥ 100 nm) and verified by SEM, TEM, optical microscopy, and laser diffraction. Available in selected sizes from 100 nm to 10 μm; full range from 20 nm to 1000 μm available on request.\u003c\/p\u003e\n\u003cp\u003eParticles are dispersed in ultrapure water at 2.5 wt% (25 mg\/mL) solids content. Sub-5 μm particles are non-crosslinked monodisperse PS; particles ≥ 5 μm are lightly crosslinked for enhanced mechanical and chemical stability.\u003c\/p\u003e\n\u003ch3\u003eWhy PolyRef™ PS Microspheres?\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTight monodispersity.\u003c\/strong\u003e CV ≤ 6% across 100 nm – 10 μm range; CV ≤ 10–20% for sub-100 nm sizes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eBatch-traceable QC.\u003c\/strong\u003e Every batch includes SEM imaging and size distribution data; COA shipped with each order.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFull size coverage on request.\u003c\/strong\u003e 20 nm to 1000 μm — over 50 size grades available for custom orders.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCited in peer-reviewed journals.\u003c\/strong\u003e RIGOR Science microspheres have been cited in \u003cem\u003eNature Water\u003c\/em\u003e, \u003cem\u003eNature Sustainability\u003c\/em\u003e, \u003cem\u003eAdvanced Science\u003c\/em\u003e, and others.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDirect from the manufacturer.\u003c\/strong\u003e No distributor markup. Custom sizes, surface modifications, and concentrations available.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eSpecifications\u003c\/h3\u003e\n\u003ctable border=\"1\" cellpadding=\"8\" cellspacing=\"0\" style=\"border-collapse: collapse; width: 100%; height: 411.469px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 39.1875px;\"\u003e\n\u003ctd style=\"height: 39.1875px;\"\u003e\u003cstrong\u003eMaterial\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 39.1875px;\"\u003ePolystyrene (PS) — non-crosslinked (≤ 5 μm) or lightly crosslinked (≥ 5 μm)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"height: 19.5938px;\"\u003e\u003cstrong\u003eCAS No.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19.5938px;\"\u003e9003-53-6\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"height: 19.5938px;\"\u003e\u003cstrong\u003eChemical Formula\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19.5938px;\"\u003e(C₈H₈)ₙ\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"height: 19.5938px;\"\u003e\u003cstrong\u003eDensity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19.5938px;\"\u003e1.05–1.07 g\/cm³\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"height: 19.5938px;\"\u003e\u003cstrong\u003eRefractive Index\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19.5938px;\"\u003e1.59 (at 589 nm)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39.1875px;\"\u003e\n\u003ctd style=\"height: 39.1875px;\"\u003e\u003cstrong\u003eSurface\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 39.1875px;\"\u003ePlain (hydrophobic); amino \/ carboxyl \/ fluorescent versions available separately\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"height: 19.5938px;\"\u003e\u003cstrong\u003eSize Uniformity (CV)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19.5938px;\"\u003e≤ 6% (100 nm – 1000 μm); ≤ 10–20% (20–80 nm)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39.1875px;\"\u003e\n\u003ctd style=\"height: 39.1875px;\"\u003e\u003cstrong\u003eAvailable Sizes (Stock)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 39.1875px;\"\u003e100 nm, 200 nm, 500 nm, 1 μm, 2 μm, 3 μm, 5 μm, 10 μm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"height: 19.5938px;\"\u003e\u003cstrong\u003eCustom Sizes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19.5938px;\"\u003e20 nm – 1000 μm available on request\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39.1875px;\"\u003e\n\u003ctd style=\"height: 39.1875px;\"\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 39.1875px;\"\u003e2.5 wt% (25 mg\/mL); 5 wt% \/ 10 wt% \/ dry powder available on request\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"height: 19.5938px;\"\u003e\u003cstrong\u003eDispersion\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19.5938px;\"\u003eUltrapure water\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"height: 19.5938px;\"\u003e\u003cstrong\u003ePackage Size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19.5938px;\"\u003e10 mL (5 mL, 50 mL, 200 mL on request)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39.1875px;\"\u003e\n\u003ctd style=\"height: 39.1875px;\"\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 39.1875px;\"\u003e2–8 °C for suspensions; room temperature (dry, dark) for powders\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"height: 19.5938px;\"\u003e\u003cstrong\u003eShelf Life\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19.5938px;\"\u003e60 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39.1875px;\"\u003e\n\u003ctd style=\"height: 39.1875px;\"\u003e\u003cstrong\u003eCharacterization\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 39.1875px;\"\u003eSEM, TEM, optical microscopy, laser diffraction; batch-specific COA included\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eApplications\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eInstrument calibration — SEM, flow cytometry, DLS, NTA, laser diffraction, optical microscopy\u003c\/li\u003e\n\u003cli\u003eParticle size reference standards and QC\u003c\/li\u003e\n\u003cli\u003ePhotonic crystal self-assembly and templating\u003c\/li\u003e\n\u003cli\u003eLCD spacer applications\u003c\/li\u003e\n\u003cli\u003eLight-scattering and diffusing agents\u003c\/li\u003e\n\u003cli\u003ePorous material templating (sacrificial templating)\u003c\/li\u003e\n\u003cli\u003eHydrodynamic and microfluidic flow tracing\u003c\/li\u003e\n\u003cli\u003eCell-size mimics for membrane and uptake studies (5–10 μm)\u003c\/li\u003e\n\u003cli\u003eDrug delivery carrier model systems\u003c\/li\u003e\n\u003cli\u003eMicroplastic spectral reference (when used alongside PVC \/ LDPE \/ PET \/ PMMA standards)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eCustom Specifications\u003c\/h3\u003e\n\u003cp\u003eNeed a size, concentration, or surface chemistry not listed above? RIGOR Science manufactures the full 20 nm – 1000 μm range and offers custom concentrations (5%, 10%, dry powder), packaging sizes (5 mL to 200 mL), and surface functionalization (amino, carboxyl, sulfate, fluorescent). Contact us with your specifications.\u003c\/p\u003e\n\u003cp\u003eSub-micron grades (100–200 nm) are characterized by DLS; micron grades (≥1 μm) are characterized by laser diffraction \/ optical microscopy. Representative data shown; see COA for lot-specific values.\u003c\/p\u003e\n\u003ch3\u003eQuality Documentation\u003c\/h3\u003e\n\u003cp\u003eEach shipment includes a batch-specific Certificate of Analysis (COA) with SEM images and size distribution data.\u003c\/p\u003e","brand":"RIGOR Science","offers":[{"title":"100 nm","offer_id":47646130012323,"sku":null,"price":79.0,"currency_code":"USD","in_stock":true},{"title":"200 nm","offer_id":47646130045091,"sku":null,"price":69.0,"currency_code":"USD","in_stock":true},{"title":"500 nm","offer_id":47646130077859,"sku":null,"price":59.0,"currency_code":"USD","in_stock":true},{"title":"1 μm","offer_id":47646130110627,"sku":null,"price":59.0,"currency_code":"USD","in_stock":true},{"title":"2 μm","offer_id":47646130143395,"sku":null,"price":59.0,"currency_code":"USD","in_stock":true},{"title":"3 μm","offer_id":47646130176163,"sku":null,"price":69.0,"currency_code":"USD","in_stock":true},{"title":"5 μm","offer_id":47646130208931,"sku":null,"price":79.0,"currency_code":"USD","in_stock":true},{"title":"10 μm","offer_id":47646130241699,"sku":null,"price":89.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0755\/0854\/7747\/files\/PS-300nm.png?v=1778504623","url":"https:\/\/rigorsci.com\/products\/monodisperse-polystyrene-ps-microspheres-size-standard-for-sem-flow-cytometry-dls-calibration-polyref%e2%84%a2","provider":"RIGOR science","version":"1.0","type":"link"}